Abstract: The aim of the study is to investigate the effects of guilt and ego depletion on self-control. In experiment 1, guilt scale, self-control scale and Stroop task were used to examine whether there was a correlation between guilt and self-control. In experiment 2, guilt priming task and E-crossing task were used to manipulate the level of guilt emotion and the degree of self-depletion, so as to examine the performance in the Stroop task. The results showed that: (1) there was a significant positive correlation between guilt and self-control; (2) when guilt was not primed, the subjects with high ego depletion showed significantly lower self-control ability than the subjects with low ego depletion; however, when guilt was primed, there was no significant difference in self-control ability between the subjects with high ego depletion and the subjects with low ego depletion. In short, in the state of ego depletion, there will be some decline in self-control, but the priming of guilt can promote self-control and alleviate the negative impact of ego depletion.